https://microbiolj.org.ua/en/archiv/2022-tom-84/6-nov-dec-tom-84/2022-84-6-02

Bacterial wetwood, bacterial dropsy, or slime is a common disease caused by Lelliottia nimipressuralis, which affects the central core of many conifers and deciduous trees. Representatives this species have been isolated from variety trees showing symptoms disease, as well water and, less commonly, clinical samples. Important aspects pathogenesis process pathogen recognition protection mechanisms cells plant’s antimicrobial substances. It known that lipopolysaccharides (LPS) take an active part in these processes. They provide barrier function outer membrane, helping to protect bacteria plant compounds, attachment cells. Therefore, aim work was study peculiarities chemical composition functional biological characteristics nimipressuralis F9a1 LPS obtained different methods. Methods. dry mass phenol-water method (LPS I), extraction with 0.85% NaCl solution II), insoluble III). The carbohydrates were analyzed Dubois method, nucleic acids ‒ Spirin, protein content Lowry 2-keto-3-deoxyoctonic acid (KDO) Osborn. identification monosaccharides fatty preparations carried out on Agilent 6890N/5973 inert chromato-mass spectrometry system. pyrogenicity tested keeping rules bioethics rabbits. Serological studies performed Ouchterlony method. Results. II L. F9а1 characterized low relative yield (2.12%), (9.16%) (3.7%), high (26.44%), while studied I III yield, rather (46.68 38.4%, respectively), insignificant amount (up 6.72%) 4.06%). All LPSs contained up 0.27% KDO, specific component gramnegative bacteria. monosaccharide indicates strains turned be heterogeneous. At same time, such fucose, galactose, glucose recorded all strains. represented presence containing 12 18 carbon atoms. Нydroxylated, saturated, monounsaturated found. In III, dominant 14:0 (3-OH), kind marker for entire family Enterobacteriaceae. addition acid, 16:1 also predominant whereas II, (32.7%) 16:0 (22.6%) dominated. pyrogenic effect showed solutions are pyrogenic. serological homologous systems exhibits antigenic activity. Antisera react IMV 8791, LGK1, L14b, may indicate determinants belonging serogroup. Conclusions. heterogeneous both composition, explained use methods their isolation. To isolate cells, water-phenol better than sodium chloride since using latter, very contaminated proteins. isolation does not affect activity LPS. results received during biological-functional contribute species.